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<title>Regeneration of plants from hypocotyl derived callus tissue of jute (Corchorus olitorius L. var. JRO-632)</title>
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<meta name="Premium Series" content="The calli were soft, loose and friable.
Some cultures also showed root formation from the
callus tissue within this period. Rhizogenesis was
observed at both the cut ends or any portion of the
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<h1 align="center" class="title style5"><span class="style1">Volume 5 Issue 2</span></h1>
<h1 align="center" class="style22">Regeneration of plants from hypocotyl derived callus tissue of jute<br />
(<em>Corchorus olitorius</em> L. var. JRO-632)<br />
<br />
</h1>
<p><span class="style21">Author(s):</span> <span class="style2">P.K.GHOSH AND A.CHATTERJEE</span><br />
<span class="style21">Abstract:</span> <span class="style3">The present paper deals with the induction and regeneration of callus tissue from in vitro system of jute (Corchrous olitorius L. Var JRO-632). Hypocotyl of in vitro germinated seedlings was taken as explants. Hypocotyl explants were cultured in both Murashige and Skoog’s (1962)(MS) and Schenk and Hildebrand’s (1972)(SH) basal media supplemented with different growth regulators in definite concentrations. Callus growth was found better in MS basal medium than SH medium. For
morphogenetic study the hypocotyl callus tissues were grown in medium containing different combinations and
concentrations of auxins (IAA, NAA, IBA and 2,4-D used separately @ 0.20 mg/L) and cytokinins (BAP and Kinetin used
separately 0.15mg/K to 3.2 mg/L, coconut milk 10-35% volume/volume). A simplified method for morphogenesis of calli was
standardized. Cytological studies of the regenerated roots from calli as well as from regenerated plants revealed diploid
chromosome number while the calli were mixoploid in nature. It was observed that the balance in the concentration of
auxin-cytokinin (in initial culture) as well as ploidy level in the cells is most important-factors in controlling differentiation.</span></p>
<p><span class="style22">Download Full Article </span><span class="style21">: </span><strong><a href="pdf/20.pdf" target="_blank">Click Here</a></strong></p>
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